3H9S
The complex between TCR A6 and human Class I MHC HLA-A2 with the bound Tel1p peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-29 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.98494 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 223.106, 48.308, 92.482 |
| Unit cell angles | 90.00, 90.97, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.221 |
| Rwork | 0.217 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gj6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.675 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.780 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.088 | 0.453 |
| Number of reflections | 27920 | |
| <I/σ(I)> | 15.186 | 1.98 |
| Completeness [%] | 99.4 | 98.2 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | PEG4000 15%, TRIS 0.1M, MgCl2 0.2M, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
