3H9P
Crystal structure of putative triphosphoribosyl-dephospho-coA synthase from Archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.806, 77.725, 66.654 |
| Unit cell angles | 90.00, 97.99, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.19713 |
| Rwork | 0.195 |
| R-free | 0.23876 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.428 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.433 |
| Number of reflections | 30320 | |
| <I/σ(I)> | 27.2 | 3.71 |
| Completeness [%] | 98.8 | 98.9 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 297 | 0.1M CHES pH 9.5, 25% PEG8000 , VAPOR DIFFUSION, SITTING DROP, temperature 297K |
