3H9M
Crystal structure of para-aminobenzoate synthetase, component I from Cytophaga hutchinsonii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | .97929 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.006, 159.497, 115.542 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.810 - 1.570 |
| R-factor | 0.174 |
| Rwork | 0.173 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| Starting model (for MR) | Built using ARP/wARP |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.279 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.370 | 1.650 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 76012 | |
| <I/σ(I)> | 19.6 | 6.4 |
| Completeness [%] | 96.7 | 78.1 |
| Redundancy | 14.4 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 100mM MES pH 6.5 + 25% PEG 3000, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
