3H92
The crystal structure of one domain of the protein with unknown function from Methanocaldococcus jannaschii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794, 0.9796 |
| Spacegroup name | P 41 |
| Unit cell lengths | 50.501, 50.501, 42.305 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.510 - 2.200 |
| R-factor | 0.20577 |
| Rwork | 0.202 |
| R-free | 0.27783 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.762 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.510 | 2.257 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.082 | 0.540 |
| Number of reflections | 5212 | |
| <I/σ(I)> | 30.46 | 2.53 |
| Completeness [%] | 99.0 | 94.49 |
| Redundancy | 9 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.05M Imidazole, 20%(w/v)PEG6000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
