3H91
Crystal structure of the complex of human chromobox homolog 2 (CBX2) and H3K27 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Collection date | 2009-01-21 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 58.145, 84.013, 65.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.500 |
| R-factor | 0.21015 |
| Rwork | 0.209 |
| R-free | 0.22299 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.461 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 2.590 | 1.200 |
| Rmerge | 0.046 | 0.022 | 0.695 |
| Number of reflections | 26030 | ||
| <I/σ(I)> | 38.995 | ||
| Completeness [%] | 93.8 | 99.2 | 61.7 |
| Redundancy | 8.1 | 9.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 30% PEG3350, 0.1 M Tris, pH8.5, 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
