3H8Z
The Crystal Structure of the Tudor Domains from FXR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Collection date | 2009-03-18 |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.563, 54.676, 70.171 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.110 - 1.920 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.729 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.960 |
| High resolution limit [Å] | 1.920 | 5.240 | 1.920 |
| Rmerge | 0.090 | 0.065 | 0.648 |
| Number of reflections | 10551 | ||
| <I/σ(I)> | 24.231 | ||
| Completeness [%] | 99.0 | 99.5 | 87 |
| Redundancy | 6.1 | 6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 10 mg/ml protein in 20 mM Tris, pH 8.0, 200 mM NaCl, 1 mM DTT; Hanging drop vapour diffusion,20-30% Peg 3350, 0.2M MgCl2, Hepes 7.5., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
