3H8S

Structure of D19N T4 RNase H in the presence of divalent magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	53.312, 75.298, 88.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.753 - 2.510
R-factor	0.192
Rwork	0.190
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1TFR native T4 RNase H
RMSD bond length	0.002
RMSD bond angle	0.450
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.035	0.098
Number of reflections	10519
<I/σ(I)>	26.4	8.9
Completeness [%]	83.1	55.2
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	100 mM Na PIPES, 200 mM MgCl2, 14% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K