3H8Q
Crystal structure of glutaredoxin domain of human thioredoxin reductase 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-27 |
| Detector | MAR225 CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 71.130, 71.130, 72.291 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.910 - 2.210 |
| R-factor | 0.19128 |
| Rwork | 0.189 |
| R-free | 0.23271 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e7p |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.330 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.910 | 2.320 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.083 | 0.951 |
| Number of reflections | 10544 | |
| <I/σ(I)> | 15.8 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.5 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 2.0M Ammonium sulphate; 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
