3H8J

Native T4 RNase H in the absence of divalent metal ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
Unit cell lengths	36.257, 86.458, 57.796
Unit cell angles	90.00, 92.04, 90.00

Refinement procedure

Resolution	19.253 - 1.800
R-factor	0.1931
Rwork	0.192
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1TFR native T4 RNase H
RMSD bond length	0.004
RMSD bond angle	0.807
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.045	0.185
Number of reflections	33128
<I/σ(I)>	28.3	7.4
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	300 mM ammonium acetate, 100 mM sodium cacodylate, 15% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K