3H8F
High pH native structure of leucine aminopeptidase from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 1 |
| Unit cell lengths | 95.786, 95.931, 96.308 |
| Unit cell angles | 68.43, 76.31, 94.86 |
Refinement procedure
| Resolution | 47.450 - 2.200 |
| R-factor | 0.19778 |
| Rwork | 0.192 |
| R-free | 0.25139 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.530 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0022) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.287 | 2.350 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.372 | |
| Number of reflections | 166778 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 96.1 | 96.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 300 | 15% (w/v) PEG 1500, 0.1 M PCB (Propionic acid, cacodylate, Bis-Tris Propane system) cocktail buffer, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
