3H86
Crystal structure of adenylate kinase from Methanococcus maripaludis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 102.840, 102.840, 228.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.700 - 2.500 |
| R-factor | 0.18821 |
| Rwork | 0.183 |
| R-free | 0.23911 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kht |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.151 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER (for MR) |
| Refinement software | PHENIX (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.690 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.082 | 0.330 |
| Number of reflections | 31158 | |
| <I/σ(I)> | 9.6 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 2.44 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 273 | 3.4 M ammonium chloride, 0.1 M sodium acetate, 3% ethylene glycol (v/v)., pH 4.7, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
