3H7Z
A transition from strong right-handed to canonical left-handed supercoiling in a conserved coiled coil segment of trimeric autotransporter adhesins - the M1 mutant structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 31.896, 31.896, 147.864 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.510 |
| R-factor | 0.23507 |
| Rwork | 0.233 |
| R-free | 0.27894 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SAD MODEL |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.828 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.003 | 0.010 |
| Number of reflections | 2590 | |
| <I/σ(I)> | 26.3 | 12 |
| Completeness [%] | 91.0 | |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 25% Peg3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
