3H7X
A transition from strong right-handed to canonical left-handed supercoiling in a conserved coiled coil segment of trimeric autotransporter adhesins - the wildtype structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.825, 105.553, 44.706 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 34.000 - 2.000 |
| R-factor | 0.25181 |
| Rwork | 0.249 |
| R-free | 0.29785 |
| Structure solution method | SAD |
| Starting model (for MR) | MODEL FROM SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.454 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.120 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | 0.360 |
| Number of reflections | 21317 | |
| <I/σ(I)> | 9.9 | 3.95 |
| Completeness [%] | 89.0 | 90 |
| Redundancy | 1.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 25% Peg3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
