3H7F
Crystal structure of serine hydroxymethyltransferase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.730, 102.010, 132.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dkj modified by ccp4 program chainsaw |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.629 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.740 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.463 |
| Number of reflections | 130938 | |
| <I/σ(I)> | 13.55 | 2.4 |
| Completeness [%] | 99.3 | 98.9 |
| Redundancy | 5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | HAMPTON INDEX, G12: 25% PEG 3350, 200MM MGCL2, 100MM HEPES, MYTUD.00783.A AT 20 MG/ML, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
