3H74
Crystal structure of pyridoxal kinase from Lactobacillus plantarum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.005, 69.649, 132.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.380 - 1.300 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.20200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.176 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.380 | 1.340 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.076 | 0.298 |
| Number of reflections | 67906 | |
| <I/σ(I)> | 13.8 | 6.3 |
| Completeness [%] | 99.5 | 98.9 |
| Redundancy | 7.8 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 298 | 1.6M Ammonium sulphate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
