3H6O
Activator-Bound Structure of Human Pyruvate Kinase M2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-02-25 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.343, 155.690, 101.789 |
| Unit cell angles | 90.00, 108.04, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.000 |
| R-factor | 0.248 |
| Rwork | 0.247 |
| R-free | 0.28700 |
| Starting model (for MR) | Direct placement of PDB entry 3GQY |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.162 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
| Rmerge | 0.077 | 0.034 | 0.947 |
| Number of reflections | 157058 | ||
| <I/σ(I)> | 11.2 | ||
| Completeness [%] | 97.0 | 99.8 | 96.8 |
| Redundancy | 3.6 | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 291 | 25% PEG3350, 0.2M ammonium acetate, 0.1M Bis-Tris, 0.005M activator, pH 5.5, vapor diffusion, temperature 291K |
