3H6H
Crystal structure of the GluR6 amino terminal domain dimer assembly MPD form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 177.253, 177.253, 81.173 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.313 - 2.901 |
| R-factor | 0.2127 |
| Rwork | 0.210 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h6g |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.753 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.089 | 0.763 |
| Number of reflections | 31930 | |
| <I/σ(I)> | 21 | 1.8 |
| Completeness [%] | 98.7 | 92.6 |
| Redundancy | 8.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 100 mM Bicine, 10% MPD, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
