3H6A
Structure of the Calx-beta domain of integrin beta4 crystallized in the presence of calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.310, 51.700, 87.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.340 - 1.608 |
| R-factor | 0.1738 |
| Rwork | 0.172 |
| R-free | 0.21250 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3fq4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.273 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 2009_02_15_2320)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.340 | 1.690 |
| High resolution limit [Å] | 1.608 | 1.608 |
| Number of reflections | 30229 | |
| <I/σ(I)> | 17.1 | 6.62 |
| Completeness [%] | 98.1 | 88.1 |
| Redundancy | 9.2 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 298 | 50MM TRIS-HCL, 2MM CACL2, 26% PEG 1500, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
