3H64
Catalytic domain of human Serine/Threonine Phosphatase 5 (PP5c) with two Mn2+ atoms complexed with endothall
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93340 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.553, 41.463, 95.614 |
| Unit cell angles | 90.00, 96.78, 90.00 |
Refinement procedure
| Resolution | 38.380 - 1.900 |
| R-factor | 0.19461 |
| Rwork | 0.190 |
| R-free | 0.23675 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s95 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.630 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.380 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.095 | 0.346 |
| Number of reflections | 47995 | |
| <I/σ(I)> | 11.9 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 10mM Tris-HCl, 40% MPD, 20% PEG MME 5000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
