3H5O
The crystal structure of transcription regulator GntR from Chromobacterium violaceum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-04-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.365, 67.882, 90.362 |
| Unit cell angles | 90.00, 92.43, 90.00 |
Refinement procedure
| Resolution | 90.170 - 2.300 |
| R-factor | 0.1913 |
| Rwork | 0.189 |
| R-free | 0.23070 |
| Structure solution method | solve |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.170 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.121 | 0.600 |
| Number of reflections | 29909 | |
| <I/σ(I)> | 14.8 | 3.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 25% w/v PEG 3350, 0.2 M Lithium sulfate, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
