3H5L
Crystal structure of a putative branched-chain amino acid ABC transporter from Silicibacter pomeroyi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.882, 121.628, 122.876 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.18500 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.337 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.442 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.434 |
| Number of reflections | 98241 | |
| <I/σ(I)> | 15.4 | 3.6 |
| Completeness [%] | 94.3 | 82 |
| Redundancy | 9.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 294 | 100mM sodium acetate pH 4.6, 25% PEG MME 550, vapor diffusion, temperature 294K |
