3H5I
Crystal structure of the N-terminal domain of a response regulator/sensory box/GGDEF 3-domain protein from Carboxydothermus hydrogenoformans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 86.668, 86.668, 131.156 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.213 |
| Rwork | 0.212 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.444 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.144 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.106 | 0.681 |
| Number of reflections | 15186 | |
| <I/σ(I)> | 14.7 | 4.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.4 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 294 | 100mM Hepes pH 8.5, 4.2M sodium chloride, vapor diffusion, temperature 294K |
