3H53
Crystal Structure of human alpha-N-acetylgalactosaminidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-05-12 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 153.532, 114.260, 68.408 |
Unit cell angles | 90.00, 96.11, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.010 |
R-factor | 0.163 |
Rwork | 0.161 |
R-free | 0.19400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ktb |
RMSD bond length | 0.007 |
RMSD bond angle | 1.048 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.080 |
High resolution limit [Å] | 2.010 | 2.010 |
Number of reflections | 77785 | |
<I/σ(I)> | 29 | 3.2 |
Completeness [%] | 99.3 | 93 |
Redundancy | 9.9 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 293 | PEG3350, citric acid, Bis-Tris propane, pH 4.1, vapor diffusion, hanging drop, temperature 293K |