3H49
Crystal structure of a putative Ribokinase (Apo Form) from E.coli at 1.8A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.088, 73.718, 82.153 |
| Unit cell angles | 90.00, 96.49, 90.00 |
Refinement procedure
| Resolution | 34.710 - 1.800 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.085 | 0.480 |
| Number of reflections | 51641 | |
| <I/σ(I)> | 10.1 | 7.7 |
| Completeness [%] | 97.9 | 96.7 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2M Ammonium Acetate,30% PEG 4K,Sodium Citrate 5.6pH, 10%Isopropanol., VAPOR DIFFUSION, SITTING DROP, temperature 293K |
