3H3Z

Crystal structure of a putative cyclic nucleotide binding protein (spoa0323) from ruegeria pomeroyi dss-3 at 2.35 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97946
Spacegroup name	H 3
Unit cell lengths	137.052, 137.052, 94.937
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.724 - 2.350
R-factor	0.204
Rwork	0.202
R-free	0.23600
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.725
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.724	28.724	2.430
High resolution limit [Å]	2.350	5.040	2.350
Rmerge	0.047	0.026	0.603
Number of reflections	27604	5386	5136
<I/σ(I)>	11.09	27.7	1.4
Completeness [%]	98.2	96	96
Redundancy	3.82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	1.2600M (NH4)2SO4, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	1.2600M (NH4)2SO4, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K, VAPOR DIFFUSION, SITTING DROP