3H3T
Crystal structure of the CERT START domain in complex with HPA-16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-03-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.340, 74.568, 78.021 |
| Unit cell angles | 90.00, 102.84, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e3n |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.469 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.074 | 0.045 | 0.340 |
| Number of reflections | 18692 | ||
| <I/σ(I)> | 26.082 | ||
| Completeness [%] | 99.9 | 99.7 | 99.5 |
| Redundancy | 5.1 | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | vapor diffusion, temperature 293K, VAPOR DIFFUSION |
