3H3L

Crystal structure of PUTATIVE SUGAR HYDROLASE (YP_001304206.1) from Parabacteroides distasonis ATCC 8503 at 1.59 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97905,0.97839
Spacegroup name	P 43 21 2
Unit cell lengths	117.259, 117.259, 118.068
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.311 - 1.590
R-factor	0.17
Rwork	0.170
R-free	0.18500
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.561
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.311	29.311	1.650
High resolution limit [Å]	1.590	3.420	1.590
Rmerge	0.064	0.010	0.010
Number of reflections	107246	19770	22016
<I/σ(I)>	16.59	48.3	1.8
Completeness [%]	96.6	92.3	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.2000M Na2Ci, 30.0000% PEG-400, 0.1M TRIS pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K