3H3A
The complex structure of CCA-adding enzyme with CTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.451, 63.586, 152.578 |
| Unit cell angles | 90.00, 103.31, 90.00 |
Refinement procedure
| Resolution | 31.793 - 2.801 |
| R-factor | 0.2352 |
| Rwork | 0.234 |
| R-free | 0.26910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h37 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.510 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 42184 | |
| <I/σ(I)> | 22.9 | 1.4 |
| Completeness [%] | 95.5 | 86 |
| Redundancy | 3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 20% ethylene glycol, 0.05M Tris-Cl pH8.4, 2mM CTP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
