3H38
The structure of CCA-adding enzyme apo form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.348, 50.455, 69.871 |
| Unit cell angles | 90.00, 91.00, 90.00 |
Refinement procedure
| Resolution | 43.330 - 2.370 |
| R-factor | 0.2142 |
| Rwork | 0.211 |
| R-free | 0.26620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h37 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.704 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.450 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Number of reflections | 23735 | |
| <I/σ(I)> | 17.1 | 2.6 |
| Completeness [%] | 95.5 | 88.5 |
| Redundancy | 2.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | 10% PEG 4000, 0.2M KCl, 5mM magnesium chloride, 0.05M HEPES pH8.1 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
