3H37
The structure of CCA-adding enzyme apo form I
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940, 0.97952, 0.98200 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.569, 62.738, 152.390 |
| Unit cell angles | 90.00, 103.96, 90.00 |
Refinement procedure
| Resolution | 48.644 - 2.850 |
| R-factor | 0.2319 |
| Rwork | 0.230 |
| R-free | 0.26650 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 40434 | |
| <I/σ(I)> | 40.2 | 3.7 |
| Completeness [%] | 97.9 | 91.7 |
| Redundancy | 5.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 20% ethylene glycol, 0.05M Tris-Cl pH8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
