3H35
Structure of the uncharacterized protein ABO_0056 from the hydrocarbon-degrading marine bacterium Alcanivorax borkumensis SK2.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940,0.97953 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.967, 82.967, 131.485 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.490 - 2.150 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.21200 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.501 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 5.830 | 2.150 |
| Rmerge | 0.093 | 0.073 | 0.678 |
| Number of reflections | 29280 | ||
| <I/σ(I)> | 37.75 | ||
| Completeness [%] | 99.9 | 98.3 | 100 |
| Redundancy | 12 | 11 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 0.2M K/Na Tartrate*4H2O, 18% PEG3340, pH 6, VAPOR DIFFUSION, temperature 291K |
