3H34
PpcE, A cytochrome c7 from Geobacter sulfurreducens
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-25 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.73859, 1.73647, 1.78875, 1.0332 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 37.700, 102.000, 22.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.320 - 1.600 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.20500 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 4.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.079 | 0.313 |
| Number of reflections | 22388 | |
| <I/σ(I)> | 28.9 | 5.5 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | Micro crystals obtained from SaltRx-31 (3.5 M sodium formate, 0.1 M bis-tris propane, pH 7.0) were optimized by micro seeding method, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
