3H33
PpcC, A cytochrome c7 from Geobacter sulfurreducens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-25 |
Detector | CUSTOM-MADE |
Wavelength(s) | 1.0332 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 79.200, 79.200, 67.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.250 |
R-factor | 0.20624 |
Rwork | 0.203 |
R-free | 0.24063 |
Structure solution method | SAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.904 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.057 | 0.314 |
Number of reflections | 19219 | |
<I/σ(I)> | 44 | 7.1 |
Completeness [%] | 99.0 | 99 |
Redundancy | 8 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 4:1 dilution of SaltRx-64 with water (2M ammonium sulfate, 0.08 M sodium acetate, pH 4.6), VAPOR DIFFUSION, HANGING DROP, temperature 298K |