3H2D

Crystal structure of a chemotactic chec-like protein (so_3915) from shewanella oneidensis mr-1 at 1.86 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-20
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162, 0.97961, 0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	58.327, 71.024, 74.039
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.606 - 1.860
R-factor	0.179
Rwork	0.177
R-free	0.21600
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.602
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.606	28.606	1.930
High resolution limit [Å]	1.860	4.000	1.860
Rmerge	0.044	0.017	0.484
Number of reflections	26303	4830	4805
<I/σ(I)>	12.93	40.2	1.97
Completeness [%]	96.7	95.1	91.1
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	NANODROP, 1.60M MgSO4, 0.1M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K