3H25

Crystal structure of the catalytic domain of primase Repb' in complex with initiator DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-05
Detector	MAR CCD 165 mm
Wavelength(s)	0.91841
Spacegroup name	P 43 21 2
Unit cell lengths	85.350, 85.350, 68.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.000 - 2.700
R-factor	0.226
Rwork	0.224
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CATALYTIC DOMAIN OF PDB 3H20 MODEL OF IDEAL B-DNA
RMSD bond length	0.005
RMSD bond angle	0.947
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.3.0040)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.000	2.770
High resolution limit [Å]	2.700	2.700
Number of reflections	7215
<I/σ(I)>	16.51	5.64
Completeness [%]	97.5	95
Redundancy	7.2	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		0.2M AMMONIUM CITRATE, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 291K