3H1T
The fragment structure of a putative HsdR subunit of a type I restriction enzyme from Vibrio vulnificus YJ016
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.244, 88.780, 114.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.390 - 2.300 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.24190 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.099 | 0.433 |
| Number of reflections | 31838 | |
| <I/σ(I)> | 11.8 | 2.42 |
| Completeness [%] | 97.6 | 87.6 |
| Redundancy | 5.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 8-10% polyethylene glycol3350, 0.15M NH4Cl, 0.1M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid at pH7.5, 2mM mercaptoethanol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
