3H1R

Order-disorder structure of fluorescent protein FP480

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	I 4 2 2
Unit cell lengths	91.394, 91.394, 53.525
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.900 - 2.410
R-factor	0.236
Rwork	0.228
R-free	0.32100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	2.105
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge		0.695
Number of reflections	4611
Completeness [%]	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	293	100mM Bis-Tris, 25% PEG 3350, 200mM MgCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K