3H15

Crystal structure of replication initiation factor MCM10-ID bound to ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 31 2 1
Unit cell lengths	95.017, 95.017, 61.159
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.138 - 2.720
R-factor	0.1991
Rwork	0.197
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ebe
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 2009_02_15_2320_3))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.720	2.720
Number of reflections	8222
<I/σ(I)>	11.38	1.55
Completeness [%]	92.6	51.4
Redundancy	5.3	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	294	100mM TAPS pH 9.0, 17% PEG 3350 , VAPOR DIFFUSION, SITTING DROP, temperature 294K
1	VAPOR DIFFUSION, SITTING DROP	9	294	100mM TAPS pH 9.0, 17% PEG 3350 , VAPOR DIFFUSION, SITTING DROP, temperature 294K