3H0U
Crystal structure of a putative enoyl-CoA hydratase from Streptomyces avermitilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.896, 102.442, 96.173 |
| Unit cell angles | 90.00, 132.11, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.17600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.278 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.611 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.098 | 0.487 |
| Total number of observations | 163832 | |
| Number of reflections | 154554 | |
| <I/σ(I)> | 14.8 | 3.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.5 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 20% PEG 3350, 200mM potassium fluoride, pH 7.0, vapor diffusion, temperature 294K |
