3H0T
Hepcidin-Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.077, 52.169, 65.630 |
| Unit cell angles | 90.00, 99.24, 90.00 |
Refinement procedure
| Resolution | 86.070 - 1.890 |
| R-factor | 0.20464 |
| Rwork | 0.202 |
| R-free | 0.25085 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8fab |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.245 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 86.070 |
| High resolution limit [Å] | 1.890 |
| Rmerge | 0.030 |
| Number of reflections | 41776 |
| <I/σ(I)> | 25.6 |
| Completeness [%] | 94.0 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100mM HEPES,70% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
