3H0N

Crystal structure of a duf1470 family protein (jann_2411) from jannaschia sp. ccs1 at 1.45 A resolution

Replaces:  2PW4

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-29
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97920,0.97879
Spacegroup name	C 1 2 1
Unit cell lengths	77.750, 59.670, 57.820
Unit cell angles	90.00, 128.79, 90.00

Refinement procedure

Resolution	25.786 - 1.450
R-factor	0.141
Rwork	0.140
R-free	0.15700
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.443
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0053)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.786	25.786	1.500
High resolution limit [Å]	1.450	3.120	1.450
Rmerge	0.028	0.013	0.249
Number of reflections	36254	6862	6767
<I/σ(I)>	14.38	40.9	2.8
Completeness [%]	97.7	93.8	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	1.40M sodium acetate, 0.10M sodium cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K