3H0K
Crystal structure of an adenylated kinase related protein from sulfolobus solfataricus to 3.25a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 65.418, 65.418, 205.437 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.300 - 3.250 |
| R-factor | 0.225 |
| Rwork | 0.221 |
| R-free | 0.29400 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.471 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.149 | 0.923 |
| Number of reflections | 8509 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 97.7 | 98.8 |
| Redundancy | 9.2 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 1.26M AMMONIUM SULFATE, 0.1M CACODYLATE PH 6.5, VAPOR DIFFUSION, TEMPERATURE 277K, VAPOR DIFFUSION, SITTING DROP |
