3H0H
human Fyn SH3 domain R96I mutant, crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-20 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 41 |
| Unit cell lengths | 41.907, 41.907, 32.764 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.950 - 1.760 |
| R-factor | 0.17012 |
| Rwork | 0.166 |
| R-free | 0.22191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shf |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.936 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.950 | 1.850 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.050 | 0.080 |
| Number of reflections | 5700 | |
| <I/σ(I)> | 25.2 | 12.3 |
| Completeness [%] | 98.5 | 92.7 |
| Redundancy | 2.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 0.5M ammonium sulphate, 0.1M sodium citrate, 1.0M lithium sulphate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
