3H08

Crystal structure of the Ribonuclease H1 from Chlorobium tepidum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-22
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.1158
Spacegroup name	P 1
Unit cell lengths	33.457, 33.388, 66.072
Unit cell angles	100.38, 99.43, 90.82

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.16146
Rwork	0.160
R-free	0.19362
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.320
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	36214
<I/σ(I)>	23.3	8.3
Completeness [%]	95.5	92.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20% PEG 4000, 0.1M sodium HEPES, 10% ispropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	20% PEG 4000, 0.1M sodium HEPES, 10% ispropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K