3H05
The Crystal Structure of a Putative Nicotinate-nucleotide Adenylyltransferase from Vibrio parahaemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-06 |
| Detector | ADSC QUANTUM 215R |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.206, 70.722, 130.495 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.600 - 1.650 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.21400 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.217 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (refmac_5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.082 | 0.731 |
| Number of reflections | 50935 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 20% PEG 3350, 0.2M Lithium chloride, vapor diffusion, temperature 277K, VAPOR DIFFUSION |
