3H04
The crystal structure of the protein with unknown function from Staphylococcus aureus subsp. aureus Mu50
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794, 0.9796 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 47.033, 47.033, 404.486 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.420 - 1.900 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.618 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.420 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.069 | 0.503 |
| Number of reflections | 20087 | |
| <I/σ(I)> | 35.9 | 3.1 |
| Completeness [%] | 94.0 | 64.8 |
| Redundancy | 9.8 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.1 | 289 | 0.2M calcium chloride dihydrate, 20% PEG3350, pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
