3GZP
HUMAN SOD1 G93A Metal-free Variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-18 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.03 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.803, 67.917, 80.962 |
| Unit cell angles | 90.00, 110.86, 90.00 |
Refinement procedure
| Resolution | 41.941 - 3.100 |
| R-factor | 0.2048 |
| Rwork | 0.203 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hl5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.958 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.4_4)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 11514 | |
| <I/σ(I)> | 8.2 | 2.6 |
| Completeness [%] | 99.3 | 95.7 |
| Redundancy | 3.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2.4 M ammonium sulfate, 0.1 M HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
