3GZM
Crystal Structure of holo PfACP Reduced Monomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-29 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54168 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 54.177, 54.177, 101.629 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.320 - 1.800 |
| R-factor | 0.20919 |
| Rwork | 0.206 |
| R-free | 0.27607 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gzl |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.112 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.320 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.199 | 0.949 |
| Number of reflections | 13930 | |
| <I/σ(I)> | 31.05 | 2.85 |
| Completeness [%] | 95.4 | 72.8 |
| Redundancy | 44.77 | 5.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 296 | 3.03M NaMalonate, 80mM BME, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
