3GZL
Crystal Structure of holo PfACP Disulfide-Linked Dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2006-01-19 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54168 |
| Spacegroup name | I 41 |
| Unit cell lengths | 48.675, 48.675, 97.588 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.560 - 2.550 |
| R-factor | 0.2418 |
| Rwork | 0.241 |
| R-free | 0.26703 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t8k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.005 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.560 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 3981 | |
| <I/σ(I)> | 12.95 | 3.14 |
| Completeness [%] | 99.6 | 98.7 |
| Redundancy | 5.64 | 4.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 3.06M NaMalonate, 2mM BME, 1mM CuCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
