3GZ2
Crystal structure of IpgC in complex with an IpaB peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.720, 113.720, 76.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.600 - 2.650 |
| R-factor | 0.235 |
| Rwork | 0.233 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gyz |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.894 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.600 | 2.800 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.069 | 0.840 |
| Number of reflections | 15633 | 1112 |
| <I/σ(I)> | 26.38 | 3.4 |
| Completeness [%] | 92.6 | 90.4 |
| Redundancy | 17.3 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1M ADA, 1M ammonium sulfate, pH6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
